Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts
نویسندگان
چکیده
منابع مشابه
Lineage analysis of inner cell mass and trophectoderm using microsurgically reconstituted mouse blastocysts.
The fate of mouse blastocyst tissues was examined following reconstitution of blastocysts from isolated inner cell mass (ICM) and trophectoderm differing for electrophoretic variants at the glucose phosphate isomerase (GPI-1) locus. A modified microsurgical method was used and a more sensitive enzyme assay allowed finer dissection of developing chimaeric conceptuses. In seven of nine cases, the...
متن کاملA novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting.
OBJECTIVE To develop a simple method to purify blastomeres of inner cell mass (ICM) and trophectoderm (TE) lineage using magnetic activated cell sorting. DESIGN Prospective laboratory study. SETTING Embryology research laboratory. PATIENT(S) None. INTERVENTION(S) Trophectoderm cells of zona-free blastocysts were labeled with concanavalin A conjugated to FITC, and every nucleus in the bl...
متن کاملDevelopment of gynogenetic and parthenogenetic inner cell mass and trophectoderm tissues in reconstituted blastocysts in the mouse.
The developmental potential of inner cell mass (ICM) and trophectoderm (TE) derived from parthenogenetic or biparental gynogenetic embryos was examined in reconstituted blastocysts with normal TE or ICM, respectively. The results demonstrate that when a normal ICM was introduced inside a trophectoderm vesicle derived from parthenogenetic or gynogenetic blastocysts, postimplantation development ...
متن کاملOct-4 expression in inner cell mass and trophectoderm of human blastocysts.
The expression of the transcription factor Oct-4 is thought to be one of the decisive factors that maintain totipotency in embryonic and germ cells. In mice, oct-4 is exclusively expressed in germ cells and totipotent cells of the embryo. In humans, Oct-4 is expressed in germ cells, embryonic stem cells and whole embryos at various stages of development. However, there is limited information ab...
متن کاملDo trophectoderm and inner cell mass cells in the mouse blastocyst maintain discrete lineages?
The extent to which trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst remain distinct during the period from the commencement of cavitation up until 48 h later in culture was investigated. Fluorescent latex microparticles were used to label exclusively all TE cells in nascent blastocysts and the position of labelled progeny in cultured blastocysts was examined by dis...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2001
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61960-8